Your sound board is 100%. I've listened to that sequence a couple hundred times. Nice to know you have 4 good Pokeys.
It sounds like something in gameplay mode is sending a reset to the sound board.
I'd also look inside the end connector for bent pins.. Make sure the finger connectors are clean on the PCBs..
View attachment 769803
Something like this 'could' cause your sound board issue..
It's also possible the reset cycle we are hearing is causing the flickering too.. is the spot killer LED blinking in the 6100?